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Objective: To analyze the processing situation of 124 batches of scorpions and optimize the salt-water processing.Methods: The character, extract, moisture, total ash, acid insoluble ash, amino acids content and heavy metals in the marketed scorpions were determined.Using extract and amino acids contents as the indices, the salt-water processing was optimized.Results: The results of the above determinations showed that the quality difference was very obvious for the marketed scorpion, and the extract and amino acids contents were the highest in scorpion boiled in 2% sodium chloride aqueous solution for 5 min.Conclusion: The item of extract test should be revised in the current standard, and the items such as moisture, total ash, acid insoluble ash, amino acids content and heavy metals should be taken into consideration in order to control the quality of scorpion more comprehensively.The best salt-water processing of scorpion is boiled in 2% sodium chloride aqueous solution for 5 min.
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Objective To determine the reliability of Chinese-version of Cerebral Palsy Quality of Life Questionnaire for Children (CPQOL) between self-raters. Methods Family members of 21 children with cerebral palsy from rehabilitation centers or hospitals, 2 mem-bers of the same one, and 18 teenagers with cerebral palsy from a special school along with one of their parents, answered CPQOL. The in-traclass correlation coefficient (ICC) between family members, as well as teenagers and their parents were calculated, and the differences of scores of each dimension between parents and teenagers were compared. Results The reliability between family members was acceptable (ICC=0.49-0.86) in all the dimension of CPQOL, which was good in the function and acquired service dimensions, and was moderate in par-ticipation and body health dimensions. The reliability was weaker between parents and teenagers (ICC=0.29-0.73), which was acceptable in the dimensions of function, and participation and body health;and very low in the dimension of welfare and acceptance. The teenagers often rated better than their parents did, especially in the dimension of participation and body health (P<0.05). Conclusion The reliability of CPQOL is acceptable when rated by family members of cerebral palsy children, especially in the dimensions of function and service ac-quired. It is weaker between parents and children, especially in the dimension of welfare and acceptance.
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Objective:To research on the immune recognition mechanism of synthetic pyrethroids and generic specific antibody.Methods:We studied on quantitative structure-activity relationship ( QSAR ) of synthetic pyrethroids and their analogs as well as antibody activity ( IC50:fifty percent inhibition concentration ) using stepwise multiple linear regression method.Based on calculating structure descriptors of synthetic pyrethroids and their analogs , two-demensional QSAR ( 2D-QSAR ) model was established.The main factors affecting antibody activity were screened using 2D-QSAR,and predictive ability of QSAR models were evaluated by the method of leave-one-out( LOO) cross-validation.Meanwhile, the structure parameters of synthetic pyrethroid fragments were calculated and then analyzed using partial least squares ( PLS) assay.And then hologram QSAR ( H-QSAR) model was constructed on molecular substructure and antibody activity.The fragments contribution to antibody activity were illustrated by encoding different colors.Results:Decision coefficent (R2) of 2D-QSAR model and HQSAR model were 0.920 and 0.917 individually,cross-validation coefficient ( Q2 ) of two QSAR models were 0.875 and 0.660 respectively ,which showed two models had good predictive abil-ity.The result from 2D-QSAR model was also obtained that smaller was hydrophobicity of pyrethroids , easier was recognized by antibody.In addition,the optimum HQSAR model was constructed after we tried many combinations of these parameters .The fragment size in optimum HQSAR model was between 4 to 10,a hologram length was 61,optimum principle component was 4,and the fragment type of B/C/Ch was selected.However ,the fingerprint encoded results of synthetic pyrethroids weren′t consistent completely with exper-imental IC50 values.Conclusion:Hydrophobicity of synthetic pyrethroids is the largest correlation factors in antibody recognization .
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Objective Signal transducers and activators of transcription 3 (STAT3) silenced by RNA interference (RNAi) technique were used to induce the apoptosis and growth inhibition in T24 and 5637 bladder cancer cells. Methods Three recombinant plasmids pGenesil-1-shRNA-STAT3 was constructed and transfected into T24 and 5637 cells. The expression of STAT3 gene was detected by RT-PCR and Western blotting. FCM was used to observe the apoptosis in T24 and 5637 cells. Results pGenesil-1-shRNA-STAT3 was successfully constructed, and transfected into T24 and 5637 cells. RT-PCR and Western blot analysis demonstrated that pGenesil-1-shRNA-STAT3 could significantly inhibit the expression of STAT3 in T24 and 5637 cells; FCM results show that it could suppress the growth of 1'24 and 5637 cells. Conclusion pGeneSiI-1-shRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of T24 and 5637 cells.
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Objective To raise ginsenosides yield from Panax ginseng by supercritical CO2 reverse microemulsion extraction.Methods Bis-(2-ethylhexyl)sodium sulphosuccinate(AOT)/ethanol/water/supercritical CO2 reverse microemulsion extraction was carried out to extract gingsenosides.Results The ginsenosides extracting rate by supercritical CO2 reverse microemulsion extraction was 3.2 times that of by ethanol/water/supercritical CO2 extraction in extracting pressure 25 MPa,extracting temperature 45 ℃,extracting time 4 h,and CO2 flow rate 2.0 L/h,respectively.The ginsenosides extracting yield increased with the increasing of the water amount and the extracting pressure,increased first and then decreased with the increasing of AOT concentration and the extracting temperature.The ginsenosides extracting yield with P.ginseng soaked with water before extraction was 1.3 times that with P.ginseng soaked with water during the extraction.Conclusion Combined the experimental results with the theoretic deduction,the mechanism of supercritical CO2 reverse microemulsion extraction is that the polar water pools of reverse microemulsion can make more ginsenosides dissolved.
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Objective:To compare the measures of activated blood platelets in different disease conditions and the effects of aspirin on blood clots.Methods:The expression of activated platelet CK62p and CD63 and its changing after taking aspirin in 126 tumor patients and 60 non-tumor patients was detected with flow cytometry.Result:The expression of activated platelet CK62p and CD63 in tumor patients was significantly higher than that in non-tumor patients(P